IN VITRO REGENERATION OF PHASEOLUS VULGARIS L. VIA ORGANOGENESIS FROM PETIOLE EXPLANTS
2005, 6 (1) p. 53-58
Margarita VELTCHEVA, Diana Lilova SVETLEVA
Abstract
A system for somatic organogenesis in common bean (Phaseolus vulgaris L.) was developed. Precultivation of seeds on different media was investigated for the ability to influence the process of in vitro regeneration. Leave petioles excised from in vitro derived seedlings at different ages (7 and 14 days-old), were used as primary explants. Precultivation of the seedlings on medium MS-BAP 1 μM for 7 days and dark cultivation of their leave petioles on medium MSI (2 μM TDZ, 0.6 μM NAA and 2 μM paclobutrazol) benefit the process of shoot initiation in all investigated genotypes. Shoot elongation took place on MSE (22.2 μM BAP and 0.057 μM IAA) medium. Plant recovery was established on MSG3 (4.44 μM BAP and 0.58 μM GA3) medium.
Keywords
сommon bean, in vitro regeneration, precultivation.
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