ANALYSIS OF PLASMA MEMBRANE INEGRITY BY USING OF FLUORESCEIN LABELED ANNEXIN V AND ITS RELATIONSHIP TO MOTILITY AND VIABILITY OF RAM SPERM AFTER CRYOPRESERVATION

2005, 6 (3)   p. 331-336

Maria IVANOVA-KICHEVA, Alexander DIMITROV, Ivan NIKOLOV, Denitza DASKALOVA, Malcho PETROV

Abstract

The present study was conducted to detect sperm apoptosis in fresh, capacitated and frozen ram semen and to determine its relationship with other seminal parameters as motility and viability. The cryopreservation process induces the plasma membrane disruptions, attended with increasing of phoshpatidylserine molecules expression on the extracellular surface, wherefore some of the live spermatozoa loss their biological potential to fertilize the egg.

Keywords

spermatozoa, cryopreservation, phosphatidylserine, annexin v.

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