DOI: https://doi.org/10.5513/JCEA01/26.3.4589
Original scientific paper
Microorganisms on granular fertilizers and prospects of their application as PGPM
2025, 26 (3) p. 667-679
Tetiana SASINA, Vitalii VOLKOGON, Anatolii Moskalenko, Liubov SHEVCHENKO, Svitlana DIMOVA, Kateryna VOLKOGON
Abstract
The study aimed to investigate the degree of microbial contamination in mineral fertilizers, assess the presence of PGPM (Plant Growth-Promoting Microorganisms) among them and evaluate the possibility of using these microorganisms for fertilizer enrichment. The number of microorganisms on fertilizer granules was determined by plating water washes on nutrient media. Bacteria were identified by morphological, physiological, biochemical properties and using the MALDI-TOF method. The effectiveness of mineral fertilizer enriched with bacteria was studied during potato cultivation on sod-podzolic soil. Bacteria were present on fertilizer granules (from 11,000 CFU/g superphosphate to 74,000 CFU/g ammonium sulfate). Micromycetes were not detected. Regardless of the type of fertilizer, representatives of Bacillus amyloliquefaciens were dominant on the granules. A small number of B. amyloliquefaciens subsp. plantarum, B. cereus and Micrococcus spp. were also detected. A total of 112 bacterial cultures were isolated. Twenty percent of isolates showed a growth-stimulating effect. Phosphate-solubilizing properties were observed in 28% of isolates, and 36% showed antagonistic activity against phytopathogenic micromycetes. Three active bacterial strains were selected for the enrichment of mineral fertilizer. NPK granules (16-16-16) were separately treated with suspensions of B. amyloliquefaciens B-22, B. cereus 3/7 and B. amyloliquefaciens subsp. plantarum 5/13. The use of biologically modified fertilizers at a dose of N80P80K80 during potato cultivation led to an intensification of the production process, increased crop yield and improved product quality. The results of the study indicate the feasibility of further studying bacteria isolated from mineral fertilizers to determine the prospects for the microbiological enrichment of fertilizers.
Keywords
potato, bio-preparations, mineral fertilizers, microorganisms, yield, sustainable agriculture
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