DOI: https://doi.org/10.5513/JCEA01/26.4.4694
Original scientific paper
Phyto-virologic status of sour cherry in Bulgaria
2025, 26 (4) p. 978-985
Abstract
This study investigated the prevalence and distribution of major viruses (PNRSV, PDV, ApMV, ACLSV, CLRV, RpRSV, and PPV) in sour cherry trees across nine regions in Bulgaria. A total of 746 sour cherry trees were sampled, comprising 534 from commercial orchards and 212 from individual trees. Samples were tested using DAS-ELISA for the presence of these viruses; ELISA for ACLSV was performed by Cocktail-ELISA. Serologically identified PNRSV and PDV were further analyzed using RT-PCR after RNA isolation from fresh leaves using a modified mini CTAB method. The overall infection rate was 14.2%, with PNRSV being the most prevalent virus (10.9%), followed by PDV (2.1%) and mixed infections (1.2%). Notably, approximately 70% of infected trees displayed no visible symptoms. No ACLSV, ApMV, PPV, CLRV, or RpRSV infection was detected in the surveyed orchards. Molecular RT-PCR analysis confirmed the presence of PNRSV and PDV at the molecular level in ELISA-positive samples. These findings underscore the importance of continuous monitoring and control measures to manage viral diseases in sour cherry orchards in Bulgaria.
Keywords
Prunus cerasus, viruses, detection, ELISA, RT-PCR
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