DOI: https://doi.org/10.5513/JCEA01/27.1.4927
Original scientific paper
Occurrence and diversity of apple mosaic ilarvirus in apple and hazelnut in Serbia
2026, 27 (1) p. 187-194
Bojana VASILIJEVIĆ, Vera KATANIĆ, Darko JEVREMOVIĆ
Abstract
Two hundred and twenty trees (150 apples and 70 hazelnuts) from seven locations in Serbia were tested for the presence of Ilarvirus ApMV (apple mosaic virus). ELISA test confirmed the presence of ApMV in 11 apples (7.3%) and six hazelnuts (8.5%) samples, with typical and severe mosaic symptoms on the leaves. ELISA-positive samples were further tested by RT-PCR with two different primer pairs. One primer pair was efficient for the RT-PCR detection of ApMV isolates from apple, and the other one for isolates from hazelnut. Sequence analysis of amplified PCR fragments of the selected isolates confirmed that Serbian ApMV isolates from apple share the highest nucleotide identity (98.66 ‒ 99.02%) with isolates from India and Canada.
Keywords
Malus × domestica Borkh., Corylus avellana L., double antibody sandwich enzyme-linked immunosorbent assay, reverse transcription polymerase chain reaction, sequencing
Download Find similar journal articles