DOI: https://doi.org/10.5513/JCEA01/27.3.5046
Original scientific paper
Periodic isolation of Xanthomonas campestris pv. campestris from soil
2026, 27 (3) p. 709-716
Slobodan VLAJIĆ, Stevan MAŠIREVIĆ, Ana VLAJIĆ, Vukašin POPOVIĆ, Janko ČERVENSKI
Abstract
Xanthomonas campestris pv. campestris (Xcc) is a major pathogen of brassicas in all production areas worldwide. The bacterium is primarily seed-borne but is also capable of persisting in soil. The duration of survival and seasonal fluctuations of soil populations play a key role in disease epidemiology. This study aimed to determine the seasonal dynamics of Xcc presence in soil under continuous cabbage monoculture. Soil sampling was conducted monthly over a two-year period (2015–2017) at the Futog locality. Samples were collected from a depth of up to 15 cm, and Xcc isolation was performed using SX selective medium. The identity of isolates was confirmed by biochemical tests and multiplex PCR analysis. The presence of the bacterium was recorded during spring (April–June), late summer (August), and autumn (September–November), whereas it was not detected during winter (December–March). This pronounced seasonal pattern indicates a clear relationship between Xcc survival in soil and temperature and moisture conditions. The results emphasize the key role of soil as an inoculum reservoir and a source of primary infections in the epidemiology of black rot in Brassicaceae.
Keywords
Xanthomonas campestris pv. campestris, soil, seasonal dynamics, inoculum, epidemiology
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