DOI: https://doi.org/10.5513/JCEA01/27.1.5053
Original scientific paper
Molecular characterization and antmicrobial susceptibility of Salmonella Enteritidis and Salmonella Typhimurium originating from the food chain
2026, 27 (1) p. 102-121
Marko DMITRIĆ, Vladimir KURĆUBIĆ, Dejan VIDANOVIĆ, Saša ŽIVKOVIĆ, Matija MUNJIĆ, Ivan VIĆIĆ, Neđeljko KARABASIL
Abstract
Salmonella Enteritidis and Salmonella Typhimurium (S. Enteritidis and S. Typhimurium) represent epidemiologically important serotypes; therefore, from the aspect of public health, their characterization is extremely important. During this research, molecular characterization and antimicrobial susceptibility testing of 60 isolates of S. Enteritidis and 60 isolates of S. Typhimurium originating from food, animal feed and poultry feces was performed. Antimicrobial susceptibility was tested using the disc diffusion method (EUCAST protocol), while the E test was used to determine the minimum inhibitory concentration (MIC). Molecular characterization was performed using the pulsed-field gel electrophoresis (PFGE) method. Confirmation was previously performed using the real-time PCR method, detecting the safA gene of S. Enteritidis and the fliA-IS200 gene of S. Typhimurium. Using the enzyme XbaI, 20 different PFGE profiles of S. Enteritidis and 21 different PFGE profiles of S. Typhimurium were determined. The results of testing 60 S. Typhimurium isolates using the disc diffusion method showed that 50% of the isolates were resistant to Ampicillin; 46.67% to Tetracycline; 31.67% to Chloramphenicol; 11.67% on Trimetoprim and 3.33% on Pefloxacin. The results of testing 60 isolates of S. Enteritidis using the disk diffusion method showed that 10% of the isolates were resistant to Pefloxacin; 5% to Ampicillin and 1.67% to Tetracycline. All resistant isolates, after the disc diffusion method, were also tested using E test strips. The results of this study justify the continued importance of Salmonella monitoring and control for the food chain to maintain food safety and protect public health.
Keywords
Salmonella, pulsed-field gel electrophoresis, disc diffusion method, antimicrobial susceptibility, E test
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