Publishers: University of Zagreb, Faculty of Agriculture, Zagreb, Croatia  |  Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Nitra, Slovakia  |  Hungarian University of Agriculture and Life Sciences, Georgikon Campus, Keszthely, Hungary  |  Agricultural University Plovdiv, Plovdiv, Bulgaria  |  University of South Bohemia, Faculty of Agriculture and Technology, České Budějovice, Czech Republic  |  Bydgoszcz University of Science and Technology, Bydgoszcz, Poland  |  University of Agricultural Sciences and Veterinary Medicine, Cluj - Napoca, Romania  |  University of Kragujevac, Faculty of Agronomy Čačak, Čačak, Serbia  |  Agricultural Institute of Slovenia, Ljubljana, Slovenia

IDENTIFICATION OF OILSEED RAPE CULTIVARS USING AFLP MARKERS

2002, 3 (4)   p. 285-292

V ČURN, J OVESNÁ, L SÁKOVÁ, R SOBOTKA

Abstract

A new type of molecular marker, fluorescence-based AFLP, was evaluated for its ability to identify oilseed rape cultivars. Each of the six tested AFLP combinations detected polymorphisms, the best combination (MCAA/ E-ACT) had 26% of polymorphic peaks from a total of 90 peaks and could distinguish analysed cultivars, and 4 out of 5 DH lines. The results presented here show that florescence-based AFLP was, for the purposes of oil seed rape cultivar fingerprinting, a very suitable approach.

Keywords

aflp, oilseed rape, brassica napus

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