THE EFFECT OF H-FABP GENE POLYMORPHISM ON CARCASS AND MEAT QUALITY IN THE POLISH NATIVE ZŁOTNICKA SPOTTED PIG
2010, 11 (4) p. 459-464
To estimate the relationships between the H-FABP/HinfI genotype and quality of carcass and meat traits, a total of 30 pigs Złotnicka Spotted breed were investigated. Blood samples were taken during slaughter into test tubes containing anticoagulant K3EDTA and stored at -25ºC until analysed. The H-FABP genotypes were identified with PCR-RFLP method using HinfI restriction endonuclease. Meat physico-chemical traits were determined in the longissimus lumborum (LL) muscle. Two alleles (H and h) and three genotypes (HH, Hh and hh) were identified within studied pig sample. The effect of H-FABP gene polymorphism was showed on body weight at slaughter and cold carcass weight, where both weights of body were higher in hh group (113.33 kg and 84.27 kg) than in Hh (106.06 kg and 76.40 kg) and HH (103.75 kg and 73.40 kg); P≤0.05 and P≤0.01. Differences between the means of backfat thickness for three genotype groups were not significant. However, significant relations were found between the polymorphism at locus H-FABP/HinfI and basic chemical meat composition. The pigs meat with genotype hh were marked by a higher intramuscular fat content (2.64%) compared to HH group (1.99%); P≤0.05. Values of all analysed traits were within ranges indicating good meat quality of the Polish native Złotnicka Spotted pigs.