DOI: https://doi.org/10.5513/JCEA01/22.2.3104

2021, 22 (2)   p. 420-428

Sahilu Ahmad RABILU, Evans Duah AGYEMANG, Bernadett FARKAS

Abstract

The use of pesticides dominates agriculture today, but unfortunately poses several threats to the environment and humans, through bioaccumulation and biomagnification. This research tested the efficacies of Salvia officinalis subsp. lavandulifolia and Salvia officinalis subsp. major aqueous extracts against Botrytis cinerea in order to curb chemical control and subsequent reduction in their threats. The leaves of Salvia officinalis subsp. lavandulifolia and Salvia officinalis subsp. major were collected in July and September 2019 and shed-dried. Then 90 ml hot distilled water was added to10 g of the crumbled leaves from each sample, left for 24 hours and the filtrate was used to prepare two different media concentrations: CC1 containing 99 ml PDA (i.e. Potato Dextrose Agar) and 11 ml extract (or ion-exchange water as control 1); and CC2 containing 88 ml PDA and 22 ml extract (or ion-exchange water as control 2). The 10 ml from each mixture (CC1, CC2 and the controls) was used as the growth media in 5 replications. Each Petri dish was centrally inoculated with 9 mm2 B. cinerea mycelia, and the area of colony was taken. All the extracts effectively inhibited the growth of Botrytis cinerea, with Som2 showing the highest inhibition. In Salvia officinalis subsp. lavandulifolia, Sol1 (July harvested) showed higher inhibition (53.90%) compared to September harvested Sol2 (40.78%) in CC2 at 3 days post inoculation (PI), while in Salvia officinalis subsp. major, Som2 (September harvested) showed higher inhibition (15.01%) compared to July harvested Som1 (0.04%) in CC1 at 7 days PI. Generally, CC2 showed higher inhibition compared to CC1.

Keywords

pesticide, mycelia, harvest, poisoned food technique, inoculation, inhibition

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